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Cellulite prevention

In order to help prevent cellulite from forming, the maturation of adipocyte must be inhibited and the size of lipid droplets must be minimized.

To test if the one active ingredient in Cellumend could both inhibit the maturation of adipocytes as well as effecting the lipid droplet size, a test was carried out on human adipocytes in order to assess the power of Cellumend in preventing cellulite.

An "in vitro" cell culture model of human pre-adipocytes was used, and it was stimulated to differentiate to adipocytes.

This was accomplished by using a potent mixture of differentiation agents which induces a strong accumulation of fat inside the lipid droplets contained in the pre-adipocytes.

The size of the lipid droplets were recorded by phase contrast microscopy and quantified by image analysis.

During analysis of the images the following parameters were quantified (average ± SD of duplicate experiments): number of cells per area number of differentiated adipocytes per area number of lipid droplets per adipocyte size of lipid droplets.

The results achieved are presented in the graphs below

Figure 1.

Effects of the active ingredient on the number of cells per area.

Figure 2.

Effects of the active ingredient on the number of adipocytes per area.

Conclusion

The number of adipocytes decreases whilst the number of total cells is not affected which means that the active ingredient in Cellumend targets specifically adipocyte maturation and that it is not cytotoxic and that it is effective in preventing cellulite.

Note

The number of cells in the negative control (non-differentiated adipocytes) is higher than in the differentiated cells (either treated with Cellumend or not) due to the differentiation process which stops proliferation. 

For the negative control there are no adipocytes, indicating no spontaneous differentiation of pre-adipocytes.

Other clinical trials

Results of tests done on cellulite

The following results were detailed after the completion of the study, and can be divided as follows:

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